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(...) For transient production of lentivirus (LV), retrovirus (RV), adeno-associated virus (AAV) or other viral vectors from producer cell lines, the sequence of plasmids used to provide vector function(s) should be verified before their use in the transient production.
(...) The use of unrelated DNA sequences, such as selection markers, that can end up in the final genetically-modified cells should be avoided unless justified. Minicircles are also beneficial in this case (...)

Lesen mehr online oder in der aktuellen Print-Ausgabe von IBI, volume 1, issue 3

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