You have decided to save time and to reduce routine work: You engage PlasmidFactory to produce your plasmids.
Final details such as order quantity / quality level of plasmid DNA, desired buffer system, desired concentration, etc. are clarified with you.
Shipment
You send a 20 µg DNA sample in a padded envelope to PlasmidFactory.
Reception QC
PlasmidFactory blinds your order by assigning a “PF-number” und creates a new database entry, in which all other steps are documented.
PlasmidFactory performs the routine reception Quality Control (QC) and then prepares a glycerol stock.
Fermentation
On the basis of the glycerol stock the fermentation will be initiated. PlasmidFactory makes use of several specific E. coli production strains.
Plasmid purification
PlasmidFactory uses optimized and multi-stage chromatographic methods for plasmid purification.
The consistently high quality and reproducibility of the DNA and thus your research results is guaranteed.
According to your individual order, further purification steps will be performed to ensure a plasmid purity at the highest level.
Product QC
PlasmidFactory prepares a detailed QC Report, that you will receive after the production.
Up to seven different quality control tests are performed by default and are already included in the price. Further QC tests, e.g. sequencing, can be arranged individually.
Shipment
You will receive the plasmid on dry ice in desired concentration and guaranteed amount.
Re-ordering
For each re-order, PlasmidFactory guarantees the same product specifications as in the initial order.
So you make sure the reproducibility of your experiments.
