Optimisation of AAV production with our exclusive, proprietary 2-Plasmid-System
PlasmidFactory is the exclusive worldwide provider of the 2-Plasmid-System for production of AAV vectors and holds the exclusive license from the German Cancer Research Centre (DKFZ) for global production, distribution, and use of Helper & Packaging plasmids of the pDG and pDP family.
We offer a large variety of Helper & Packaging plasmids for the production of serotype-specific AAV vectors. These plasmids are available from our In-Stock service.
Advances in the field of gene therapy have demonstrated the potential of viral vectors to treat various monogenic, inherited diseases in human patients. Thanks to their unique characteristics, recombinant vectors derived from adeno-associated viruses (AAVs) represent a particularly effective technology for delivering therapeutic genes to their targets. Optimizing production of these recombinant AAV vectors to meet the demand for increasingly large quantities, while maintaining quality and cost-effectiveness, is a key challenge.
Our In-Stock service currently includes Helper & Packaging plasmids to produce serotypes 1-6, 8, and 9. We offer complete characterization of your DNA by LPS assay, BCA assay, UV spectroscopy, restriction analysis, and detection of RNA and bacterial chromosomal DNA by agarose gel electrophoresis.
These plasmids are also available on request in HQ (High Quality) grade for clinical applications.
In-Stock:
Plasmids & Minicircles for AAV production
Helper & Packaging/Vector constructs
All In-Stock products are pre-produced in Research grade quality and are therefore available at short notice. Minimum order quantity 5 mg (plasmid DNA) or 0.5 mg (minicircle DNA). Other quality grades on request.
| Item No. | Name | Comment | Size/bp |
|---|---|---|---|
Item No. PF0400 | Name pDM | Comment Serotype: rep2/cap2 wild type: rep (rep89, 68, 52, 49) ITR of AAV2, no RFP | Size/bp 21525 bp |
Item No. PF0401 | Name pDP1rs | Comment Serotype: rep2/cap1 RFP | Size/bp 23682 bp |
Item No. PF0402 | Name pDP2rs | Comment Serotype: rep2/cap2 RFP | Size/bp 23661 bp |
Item No. PF0403 | Name pDP3rs | Comment Serotype: rep2/cap3 RFP | Size/bp 23704 bp |
Item No. PF0404 | Name pDP4rs | Comment Serotype: rep4/cap4 RFP | Size/bp 23072 bp |
Item No. PF0405 | Name pDP5rs | Comment Serotype: rep2/cap5 RFP | Size/bp 23655 bp |
Item No. PF0406 | Name pDP6rs | Comment Serotype: rep2/cap6 RFP | Size/bp 23745 bp |
Item No. PF0431 | Name pDP1 | Comment Serotype: rep2/cap1 like pDP1rs, no RFP | Size/bp 21855 bp |
Item No. PF0421 | Name pDG (=pDP2) | Comment Serotype: rep2/cap2 like pDP2rs, no RFP | Size/bp 21829 bp |
Item No. PF0433 | Name pDP3 | Comment Serotype: rep2/cap3 like pDP3rs, no RFP | Size/bp 21876 bp |
Item No. PF0434 | Name pDP4 | Comment Serotype: rep4/cap4 like pDP4rs, no RFP | Size/bp 21234 bp |
Item No. PF0435 | Name pDP5 | Comment Serotype: rep2/cap5 like pDP5rs, no RFP | Size/bp 21828 bp |
Item No. PF0436 | Name pDP6 | Comment Serotype: rep2/cap6 like pDP6rs, no RFP | Size/bp 21913 bp |
Item No. PF0478 | Name pDP8.ape | Comment Serotype: rep2/cap8 | Size/bp 21991 bp |
Item No. PF0439 | Name pDP9 | Comment Serotype: rep2/cap9 | Size/bp 21839 bp |
Item No. PF0422 | Name pDG (R484E/R585E) | Comment Serotype: rep2/cap2 like pDG (PF0421), but without HSGAG binding motif | Size/bp 19153 bp |
Item No. PF1451 | Name pAAV-ssGFP | Comment vector plasmid with AAV-ITRs, CMV promotor, GFP, selection marker (Amp or Kan) | Size/bp 7956 bp |
Item No. PF1500 | Name pAAV-scGFP | Comment vector plasmid with AAV-ITRs, CMV promotor, GFP, selection marker (Amp or Kan) | Size/bp 5964 bp |
Item No. MC3051 | Name MC.AAV-ssGFP | Comment AAV-ITRs, CMV promotor, GFP, no bacterial backbone (e.g., no resistance genes) | Size/bp 4512 bp |
Item No. MC3064 | Name MC.AAV-scGFP | Comment AAV-ITRs, CMV promotor, GFP, no bacterial backbone (e.g., no resistance genes) | Size/bp 2473 bp |
The 2-Plasmid-System for AAV production offers a cost-effective solution, maximizing efficiency and minimizing expenses.
PlasmidFactory has obtained an exclusive, worldwide license for the production, distribution, and use of the Helper & Packaging plasmids of the pDG and pDP families from the German Cancer Research Centre (DKFZ).
AAV vector plasmids – keeping ITRs intact
PlasmidFactory’s proprietary ITRRESCUE® technology enables amplification of plasmid DNA containing sensi-tive inverted terminal repeats (ITR). While restriction digestion with SmaI is the standard quality control for ITR sequences, this method is not 100% accurate and does not allow quantification of the fraction of non-intact plasmids. PlasmidFactory’s self-developed Next Generation Sequencing (NGS) technology complements our fermentation process, allowing sequencing and quantification of ITR sequences while maintaining intact plasmids. GFP reporter gene cassettes with ITR2 flanked single strandet (ss) or self-complementary (sc) reporter genes are available In-Stock as minicircle or plasmid DNA.
(Fig. artistic representation)
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Gene Delivery Vehicles – AAVs for long-term gene expression
Adeno-associated viruses have great potential in the field of gene therapy. The use of minicircles improves the quality of viral vector preparations and increases production efficiency.
When Minicircles were used for AAV production we got an increase of the genomic titer between 300-400% and an increase of the yield per cell.
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